Efeito da ingestão de álcool durante a lactação e após o desmame sobre o metabolismo hepático e cerebral da cria: um estudo em ratos

Efeito da ingestão de álcool durante a lactação e após o desmame sobre o metabolismo hepático e cerebral da cria: um estudo em ratos

Título alternativo Effect of ethanol ingestion during lactation and after weaning on offspring liver and brain metabolism in rats
Autor Oyama, Lila Missae Autor UNIFESP Google Scholar
Orientador Nascimento, Claudia Maria Oller do Autor UNIFESP Google Scholar
Resumo This study evaluated the effect of ethanol ingestion by lactating and weaned rats on liver and brain metabolismo For this, female Wistar rats, at the first day post partwn, were kept with 4 female and 4 male pups and were divided in two groups: E - received 4 por cento ethanol liquid diet and C - received normal isocaloric liquid diet. On day 24 of life, half of the female (F) and male (M) pups were decapitated. Half of then were divided in four groups: MEC and FEC -received ethanol during lactation and normal diet after weaned; MCE and FCE - received normal diet during lactation and ethanol diet after weaned; MEE and FEE - received ethanol diet during the experimental period; MCC and FCC - received normal diet during experimental period. These animais were killed at 44 days of life. Body weight (g) were measured during all the experimental period. Food consumption was determinei after weaned (kcal/ 100 g of body weight). Ethanol intake, during lactation and/or after weaned, decreased body weight in male and female rats. After weaned food intake was decreased in ethanol treated rats. The relative weight (g/lOOg b. w.) of liver and brain was increased in male rats, with 44 days of life that received ethanol during lactation and/or after weaned. On the other hand, in FEE the liver relative weight was decreased and brain relative weight was increased only in FCE and FEE. Liver protein content was increased in MCE, MEE, FCE and FEE. At 24 days of life ethanol treatment decreased liver ATP citrate lyase activity in male and malic enzyme in male and female. ln female rats that received normal diet after weaned (FEC) the activities of these enzymes were normalized. Although in MEC ATP cit activity increased in liver. Liver lipogenesis rate was increased in MCE, MEE and FEE. Brain relative weight, from male and female ethanol treated rats with 24 days of life, were increased and no changes in enzyme activities were detected. At 44 days of life brain ATP cit activity were decreased in MEE and FEC and malic enzyme in MEC, FCE and FEC. On the other hand in FEE it was observed an increase in ATP cit and malic enzyme activities in this tissue. Brain glucose metabolism and lactate production were studied in male and female rats with 24 or 44 days of life. For these brain slices were incubated at 37ºC in Krebs-Henseleit buffer under carbogen, in the presence of glucose (5mM) plus l4C-glucose (O.04 mCi) with O-hydroxybutyrate or insulin...(au)
Assunto Lactação
Etanol
Fígado
Cérebro/metabolismo
Idioma Português
Data 2000
Publicado em São Paulo: [s.n.], 2000. 100 p. tab.
Editor Universidade Federal de São Paulo (UNIFESP)
Extensão 100 p.
Direito de acesso Acesso restrito
Tipo Tese de doutorado
URI http://repositorio.unifesp.br/handle/11600/16863

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