Adult bone marrow-derived mononuclear cells expressing chondroitinase AC transplanted into CNS injury sites promote local brain chondroitin sulphate degradation

Adult bone marrow-derived mononuclear cells expressing chondroitinase AC transplanted into CNS injury sites promote local brain chondroitin sulphate degradation

Autor Coulson-Thomas, Yvette May Autor UNIFESP Google Scholar
Coulson-Thomas, Vivien Jane Autor UNIFESP Google Scholar
Filippo, Thais R. Autor UNIFESP Google Scholar
Mortara, Renato A. Autor UNIFESP Google Scholar
Silveira, Rafael B. da Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Porcionatto, Marimelia A. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Injury to the CNS of -vertebrates leads to the formation of a glial scar and production of inhibitory molecules, including chondroitin sulphate proteoglycans. Various studies suggest that the sugar component of the proteoglycan is responsible for the inhibitory role of these compounds in axonal regeneration. By degrading chondroitin sulphate chains with specific enzymes, denominated chondroitinases, the inhibitory capacity of these proteoglycans is decreased. Chondroitinase administration involves frequent injections of the enzyme at the lesion site which constitutes a rather invasive method. We have produced a vector containing the gene for Flavobacterium heparinum chondroitinase AC for expression in adult bone marrow-derived cells which were then transplanted into an injury site in the CNS. the expression and secretion of active chondroitinase AC was observed in vitro using transfected Chinese hamster ovarian and gliosarcoma cells and in vivo by immunohistochemistry analysis which showed degraded chondroitin sulphate coinciding with the location of transfected bone marrow-derived cells. Immunolabelling of the axonal growth-associated protein GAP-43 was observed in vivo and coincided with the location of degraded chondroitin sulphate. We propose that bone marrow-derived mononuclear cells, transfected with our construct and transplanted into CNS, could be a potential tool for studying an alternative chondroitinase AC delivery method. (c) 2008 Elsevier B.V. All rights reserved.
Assunto chondroitinase AC
chondroitin sulphate
bone marrow-derived cells
injury
nervous system
Idioma Inglês
Data 2008-06-15
Publicado em Journal of Neuroscience Methods. Amsterdam: Elsevier B.V., v. 171, n. 1, p. 19-29, 2008.
ISSN 0165-0270 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 19-29
Fonte http://dx.doi.org/10.1016/j.jneumeth.2008.01.030
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000256392400003
URI http://repositorio.unifesp.br/handle/11600/30733

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