An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

Autor Antonioli, Eliane Google Scholar
Lobo, Anderson O. Google Scholar
Ferretti, Mario Autor UNIFESP Google Scholar
Cohen, Moises Autor UNIFESP Google Scholar
Marciano, Fernanda R. Google Scholar
Corat, Evaldo J. Google Scholar
Trava-Airoldi, Vladimir J. Google Scholar
Instituição Hosp Israelita Albert Einstein
Univ Vale Paraiba
Universidade Federal de São Paulo (UNIFESP)
Inst Nacl Pesquisas Espaciais
Resumo Cartilage serves as a low-friction and wear-resistant articulating surface in diarthrodial joints and is also important during early stages of bone remodeling. Recently, regenerative cartilage research has focused on combinations of cells paired with scaffolds. Superhydrophilic vertically aligned carbon nanotubes (VACNTs) are of particular interest in regenerative medicine. the aim of this study is to evaluate cell expansion of human articular chondrocytes on superhydrophilic VACNTs, as well as their morphology and gene expression. VACNT films were produced using a microwave plasma chamber on Ti substrates and submitted to an O-2 plasma treatment to make them superhydrophilic. Human chondrocytes were cultivated on superhydrophilic VACNTs up to five days. Quantitative RT-PCR was performed to measure type I and type II Collagen, Sox9, and Aggrecan mRNA expression levels. the morphology was analyzed by scanning electron microscopy (SEM) and confocal microscopy. SEM images demonstrated that superhydrophilic VACNTs permit cell growth and adhesion of human chondrocytes. the chondrocytes had an elongated morphology with some prolongations. Chondrocytes cultivated on superhydrophilic VACNTs maintain the level expression of Aggrecan, Sox9, and Collagen II determined by gPCR. This study was the first to indicate that superhydrophilic VACNTs may be used as an efficient scaffold for cartilage or bone repair. (C) 2012 Elsevier B.V. All rights reserved.
Assunto Multiwalled carbon nanotubes
Vertically aligned
Superhydrophilic
Endochondral ossification
Chondrocytes
Gene expression
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento FAPESP: Proc: 2011/17877-7
FAPESP: 2011/20345-7
Data 2013-03-01
Publicado em Materials Science & Engineering C-materials for Biological Applications. Amsterdam: Elsevier B.V., v. 33, n. 2, p. 641-647, 2013.
ISSN 0928-4931 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 641-647
Fonte http://dx.doi.org/10.1016/j.msec.2012.10.010
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000315761700011
URI http://repositorio.unifesp.br/handle/11600/36037

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