USE of RETINYL ACETATE, RETINOIC ACID and INSULIN-LIKE GROWTH FACTOR-I (IGF-I) TO ENHANCE GOAT EMBRYO PRODUCTION

USE of RETINYL ACETATE, RETINOIC ACID and INSULIN-LIKE GROWTH FACTOR-I (IGF-I) TO ENHANCE GOAT EMBRYO PRODUCTION

Autor Chiamenti, Adauto Google Scholar
Aguiar Filho, Cristiano Rocha Google Scholar
Moura, Marcelo Tigre Google Scholar
Paula-Lopes, Fabiola Freitas Autor UNIFESP Google Scholar
Neves, Jairo Pereira Google Scholar
Cavalcanti Neto, Cicero Cerqueira Google Scholar
Dias Goncalves, Paulo Bayard Google Scholar
Lima, Paulo Fernandes Google Scholar
Lemos Oliveira, Marcos Antonio Google Scholar
Instituição Univ Fed Rio Grande do Norte
Univ Fed Rural Pernambuco UFRPE
Universidade Federal de São Paulo (UNIFESP)
Universidade de Brasília (UnB)
Univ Fed Alagoas
Universidade Federal de Sergipe (UFS)
Resumo Experiments were carried out to investigate the beneficial effects of retinyl acetate (RAc) and retinoic acid (RA) on goat oocyte maturation as well as the effects of insulin-like growth factor-I (IGF-I), RAc and RA during embryo culture under chemically defined conditions. in Experiment 1, in vitro maturation (IVM) was performed in a chemically defined basic maturation medium (bMM) supplemented with 0.3 mu M RAc or 0.5 mu M RA. Presumptive zygotes and embryos (24 cells) were cultured in droplets of potassium simplex optimised medium (KSOM); however, none of the embryos reached the blastocyst stage. in Experiment 2, oocytes were matured in bMM + RAc or bMM + RA. Presumptive zygotes and 2- to 4-cell embryos were placed in fresh KSOM droplets supplemented with RAc, RA, IGF-I, RAc+IGF-I or RA+IGF-I. in Experiment 1, addition of RAc and RA to bMM increased (P < 0.05) the proportion of 2- to 4-cell embryos reaching the morula stage as compared to the control. in Experiment 2, supplementation of embryo culture media with retinoids and IGF-I increased (P < 0.05) the proportion of 2- to 4-cell stage embryos developing to the morula and blastocyst stage. Our data demonstrate that goat embryo production in chemically defined media could be improved by exogenous RAc or RA and by the interaction between retinoids and IGF-I, and that goat embryos can be produced in vitro from oocytes following protocols similar to those currently used for cattle.
Assunto Vitamin A
retinyl acetate
retinoic acid
insulin-like growth factor-I
morula
blastocyst
goat embryo
production
Idioma Inglês
Data 2013-03-01
Publicado em Acta Veterinaria Hungarica. Budapest: Akademiai Kiado Rt, v. 61, n. 1, p. 116-124, 2013.
ISSN 0236-6290 (Sherpa/Romeo, fator de impacto)
Editor Akademiai Kiado Rt
Extensão 116-124
Fonte http://dx.doi.org/10.1556/AVet.2012.058
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000315236400012
URI http://repositorio.unifesp.br/handle/11600/36071

Mostrar registro completo




Arquivos deste item

Arquivos Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item aparece na(s) seguinte(s) coleção(s)

Buscar DSpace


Navegar

Minha conta