Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography

Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography

Autor Sugawara, Eduardo Kinio Autor UNIFESP Google Scholar
Ribeiro Neto, Luciane Maria Autor UNIFESP Google Scholar
Verreschi, Ieda Therezinha do Nascimento Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.
Assunto HPLC
cortisol
urine
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento CNPq: 478243/2004-9
Data 2010-01-01
Publicado em Química Nova. Sociedade Brasileira de Química, v. 33, n. 2, p. 447-450, 2010.
ISSN 0100-4042 (Sherpa/Romeo, fator de impacto)
Editor Sociedade Brasileira de Química
Extensão 447-450
Fonte http://dx.doi.org/10.1590/S0100-40422010000200036
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000275268400036
SciELO S0100-40422010000200036 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/5568

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