Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke

Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke

Autor Valenti, Vitor Engrácia Autor UNIFESP Google Scholar
Abreu, Luiz Carlos de Google Scholar
Fonseca, Fernando Luiz Affonso Autor UNIFESP Google Scholar
Adami, Fernando Google Scholar
Sato, Monica Akemi Google Scholar
Vanderlei, Luiz Carlos Marques Google Scholar
Ferreira, Lucas Lima Google Scholar
Rodrigues, Luciano M. Google Scholar
Ferreira, Celso Autor UNIFESP Google Scholar
Instituição Universidade Estadual Paulista (UNESP)
Faculdade de Medicina do ABC Departamento de Morfologia e Fisiologia
Universidade Federal de São Paulo (UNIFESP)
Resumo OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth cerebral ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.
Assunto Oxidative Stress
Catalase
Medulla Oblongata
Tobacco
Air Pollutants
Idioma Inglês
Financiador Foundation of Support to Research of Sao Paulo State (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP)
Número do financiamento 10/17769-7
Data 2013-06-01
Publicado em Clinics. Faculdade de Medicina / USP, v. 68, n. 6, p. 851-857, 2013.
ISSN 1807-5932 (Sherpa/Romeo, fator de impacto)
Editor Faculdade de Medicina / USP
Extensão 851-857
Fonte http://dx.doi.org/10.6061/clinics/2013(06)21
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000320463800021
SciELO S1807-59322013000600851 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/7836

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